Effects of dietary biotin supplement on growth, body composition, intestinal enzyme activities and microbiota of juvenile Jian carp (Cyprinus carpio var. Jian)

Abstract

A total of 1400 juvenile Jian carp (Cyprinus carpio var. Jian) (7.72 ± 0.02 g) were fed seven purified diets containing 0.010 (basal diet), 0.028, 0.054, 0.151, 0.330, 1.540 and 2.680 mg biotin kg−1 for 63 days to investigate the effects of biotin on growth, body composition, intestinal enzyme activities and microbiota. Specific growth rate (SGR), feed intake, feed efficiency and protein retention value were the highest when dietary biotin level was 0.151 mg kg−1 diet. Crude protein, lipid and ash content of fish carcass improved with increasing dietary biotin levels up to 0.054, 0.151 and 0.028 mg kg−1 diet, respectively (P < 0.05). Intestinal folds height, trypsin, chymotrypsin, lipase, amylase, alkaline phosphatase, Na+, K+-ATPase, γ-glutamyl transpeptidase and creatinekinase activities increased with dietary biotin levels up to 0.151–0.330 mg kg−1 diet (P < 0.05). Intestinal Aeromonas and Escherichia coli significantly decreased with increasing dietary biotin up to 0.151 mg kg−1 diet, while Lactobacillus and Bacillus significantly increased with dietary biotin levels up to 0.054 and 0.151 mg kg−1 diet, respectively. In conclusion, biotin could improve digestive and absorptive ability of fish, and the dietary biotin requirement for SGR of juvenile Jian carp (7.72–32.67 g) was 0.15 mg kg−1 diet.