Abstract
A 4-week feeding trial was conducted to assess the effect of AA on growth performance, hematological parameters, antioxidant and non-specific immune response in starry flounder, Platichthys stellatus (mean weight, 139.47 ± 1.41 g; mean length, 19.87 ± 0.21 cm). AA diets were designed as 0, 50, 100, 200, 400, 600 and 800 mg/kg, supplied as l-ascorbyl-2-monophosphate (AMP). The results of present study indicated that body weight gain (BWG), feed efficiency ratio (FER) and specific growth rate (SGR) were significantly higher than control group fed AA-free diet. In addition, AA diet increased red blood cell (RBC) count, hematocrit (Ht), hemoglobin (Hb), plasma calcium and magnesium, whereas total protein, glutamic oxaloacetic transaminase (GOT) and glutamic pyruvate transaminase (GPT) were significantly decreased. The superoxide dismutase activity (SOD), catalase activity (CAT), glutathione (GSH) and glutathione-S-transferase (GST) activity were significantly decreased compared with control group. Lysozyme and phagocytosis were significantly enhanced by increasing dose of AA supplementation. These results indicated that AA diet had a positive effect on growth performance, hematological parameter, antioxidant and non-specific immunity in starry flounder.
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