Effects of dietary alpha-tocopheryl acetate on lipid oxidation and alpha-tocopherol content of novel omega-3-enhanced farmed rainbow trout ( Oncorhynchus mykiss) fillets

Abstract

A trout diet was supplemented with 0, 8.5, or 15 g/100 g of flaxseed oil (FO). To prevent lipid oxidation of fillets, FO-supplemented diets were also enhanced with 0, 400, and 900 mg/kg of alpha-tocopheryl acetate ( α-TA). Total fat, moisture content, lipid oxidation, fatty acid profile, and α-tocopherol content of fillets were determined following fish harvest on days 0, 30, 60, 90, and 120. FO supplementation resulted in increased ( P<0.05) concentration of omega-3 fatty acid (ω3 FA) in fillets, mainly due almost two-fold increase ( P<0.05) of α-linolenic acid, while docosahexaenoic and eicopentaenoic acids slightly decreased ( P<0.05). Regardless of supplementing trout diets with FO or α-TA, no ( P>0.05) difference of the total fat in fillets was measured. The highest ( P<0.05) α-tocopherol content in fillets was determined when supplementing trout with 900 mg/kg of α-TA at day 120. The effect of retarding lipid oxidation in fillets was recorded after supplementing trout with α-TA for 60 days. Our results indicate that regardless of FO level in trout diet, 900 mg/kg of α-TA can prevent lipid deterioration of fillets. However, to achieve more pronounced antioxidant effect in the ω-3-enhanced trout fillets, a synergetic effect of antioxidants and anaerobic packaging with α-TA supplementation should be investigated.