Abstract
A lipotrope-deficient, high-fat diet that enhances aflatoxin B 1 (AFB 1) hepatocarcinogenesis in rats influenced in two ways the conversion of AFB 1 to mutagens detectable in the Ames Salmonella mutagenesis test system. First, in the urine of rats fed the lipotrope-deficient diet and given a single dose of AFB 1, the proportion of mutagenic material that did not require activation by hepatic microsomes was higher than in the urine of rats fed an adequate diet. Second, hepatic microsomal preparations (S-9 fractions) from deficient animals showed significantly lower activity in the in vitro converstion of AFB 1 to mutagen(s) than did S-9 fractions from normal animals. The S-9 fractions from deficient animals actually suppressed the activity of normal S-9 fractions in a mixed mutagenesis assay. The inconsistency of the second finding with the enhanced sensitivity of deficient animals to AFB 1 carcinogenesis sugggests the need for some caution in using the in vitro mutagenesis tests for quantitative estimation of carcinogenic potency.
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