Effects of dichromate on growth and root system architecture of Arabidopsis thaliana seedlings

Abstract

The hexavalent form of chromium [Cr(VI)] is toxic for most organisms; however, very little information is available regarding the effects of this metal on plant morphogenesis. In this work, we investigated the effects of Cr(VI) on the growth and development of Arabidopsis thaliana, a species widely used as a model for studying the diverse physiological and cellular processes in plants. Elongation of root hairs and biomass production were stimulated by relatively low concentrations (100 μM) of Cr(VI) as potassium dichromate. Concentrations of Cr(VI) greater than 200 μM were toxic to plants as revealed both by arrested growth of roots and shoots and the development of chlorosis in leaves. At 200 μM the primary root growth was totally inhibited but the plants continued their growth manifesting different alterations in root development. These alterations correlated with changes in mitotic activity and in cellular expansion. The analyses of A. thaliana transgenic plants that express the auxin-inducible marker DR5:uidA, and the response of the auxin-resistant mutants axr2 and aux1–7 to dichromate suggest that auxins do not participate as mediators in the cellular and physiological responses to this metal. The primary root growth inhibition by 200 μM dichromate was alleviated by more than 70% by increasing the sulfate, phosphate or nitrate concentration in the media, which suggests a relation of dichromate with these mineral nutrients.