Effects of Dichloromethane Fraction of Phlomidis Radix on Bone Formation in Human Fetal Osteoblasts

Abstract

【The ideal goal of periodontal therapy is the regeneration of periodontal tissue repair of function. Although is very difficult to attain the goal, recent advances in periodontal wound healing concepts encourage hope reaching it. Recently many efforts are concentrated on the regeneration potential of material used in traditional Korean medicine. Phlomidis Radix has been used for the treatment of blood stasis, bone fracture and osteoporosis in traditional Korean medicine. The purpose of this study is to examine effects of dichloromethane fraction Phlomidis Radix on Bone Formation in Human Fetal Osteoblasts. Human fetal osteoblastic cell line(hFOB1 1.19 ;American Type Culture Collection, Manassas, VA) were used and cells were cultured containing DMEM and dichloromethane fraction Phlomidis Radix(100 ng/ml , 1 ${\mu}$ /ml, 10 ${\mu}$ /ml) at 34 $^{\circ}C$ with 5% $CO_2$ in 100% humidity. MTT was performed to examine the viability of the cell, and alkaline phosphatase activity was analyzed to examine the mineralization. Also bone calcification nodules were evaluated. The cellular activity of hFOB1 was increased in 100 ng/ml, 1 ${\mu}$ /ml , 10 ${\mu}$ /ml of dichloromethane fraction of Phlomidis Radix and especially significant increation was showed in 100 ng/ml of dichloromethane fraction of Phlomidis Radix at 6days (p ${\mu}$ /ml, 10 ${\mu}$ /ml of dichloromethane fraction of Phlomidis Radix and especially more increation was showed in 10 ${\mu}$ /ml of dichloromethane fraction of Phlomidis Radix (p ${\mu]$ /ml of dichloromethane fraction of Phlomidis Radix at 21 days of incubation(p】