Effects of diaspirin-cross-linked hemoglobin (dclhb tm) on local tissue oxygen tension in striated skin muscle: An efficacy study in the hamster

Abstract

Using the dorsal skin fold chamber model in the hamster, we analyzed local tissue partial oxygen pressure (PO 2) in the striated skin muscle under nonischemic and postischemic conditions with a Clark-type multiwire oxygen surface electrode. Hypervolemic infusion (500 mg · kg −1 IV) or isovolemic exchange transfusion (3.3 gm · kg −1 IV; hematocrit 30%) with diaspirin-cross-linked hemoglobin (DCLHb) resulted in a slight decrease of the mean value of the local tissue PO 2 (mm Hg) 1 hour after administration. Concomitantly, the frequency distribution curves of local tissue PO 2 values were found to be more narrow (fewer values > 25 mm Hg and < 10 mm Hg). Resuscitation from severe hemorrhagic shock (bleeding of 33 ml · kg −1 at 0.4 ml · min −1) with autologous blood (AuB), Dx-60, or DCLHb TM led to an increase of mean tissue PO 2 values by 4.2-fold ( p < 0.05 versus Dx-60), 1.9-fold, and 3.7-fold ( p < 0.05 versus Dx-60), respectively, 2 hours after resuscitation. The reduction of tissue hypoxia (0-5 mm Hg) was significant only in the AuB- and DCLHb TM-treated animals. This study indicates that DCLHb TM effectively reverses tissue hypoxia after resuscitation from severe hemorrhagic shock by inducing a more homogeneous distribution of the local tissue PO 2 levels.