Abstract
Objective To evaluate the effect of dexmedetomidine pretreatment on the expression of mitochondrial transcription factor A (TFAM) and succinate dehydrogenase (SDHA) during lung ischemia-reperfusion (I/R) in mice. Methods Twenty-four clean-grade healthy male C57BL/6J mice, aged 8-10 weeks, weighing 18-22 g, were divided into 3 groups (n=8 each) using a random number table method: sham operation group (group S), lung I/R group (group I/R) and dexmedetomidine pretreatment group (group D). In I/R and D groups, lung I/R was induced by clamping the left pulmonary hilum for 60 min followed by 120-min reperfusion in anesthetized mice.The chest was only opened, but the left pulmonary hilum was not occluded in group S. Dexmedetomidine 25 μg/kg was intraperitoneally injected at 30 min before ischemia in group D, while the equal volume of normal saline was given instead of dexmedetomidine in I/R and S groups.The mice were sacrificed at 120 min of reperfusion, and lungs were removed for determination of wet/dry weight ratio (W/D ratio), cell apoptosis (by TUNEL) and expression of TFAM and SDHA mRNA in lung tissues (by real-time polymerase chain reaction) and for examination of the pathological changes of lung tissues.The apoptosis index was calculated. Results Compared with group S, the W/D ratio, apoptosis index and lung injury scores were significantly increased, and the expression of TFAM and SDHA mRNA was down-regulated in I/R and D groups (P<0.05). Compared with group I/R, the W/D ratio, apoptosis index and lung injury scores were significantly decreased, and the expression of TFAM and SDHA mRNA was up-regulated in group D (P<0.05). Conclusion The mechanism by which dexmedetomidine pretreatment attenuates lung I/R injury is related to up-regulating the expression of TFAM and SDHA in mice. Key words: Dexmedetomidine; Lung; Reperfusion injury; Mitochondria; Transcription factors; Succinate dehydrogenase
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