Effects of derivatives of hydroxypyruvaldehyde phenylosazone on bovine erythrocyte membrane. I. Influence on the osmotic fragility and morphology.

Abstract

Thirteen derivatives of hydroxypyruvaldehyde phenylosazone were tested for effects on bovine erythrocyte membrane. Six drugs, including hydroxypyruvaldehyde phenylosazone itself and the o-CH3, m-CH3, p-CH3, o-Cl and p-Cl derivatives, increased the stability of bovine erythrocyte membrane to hypotonic shock ; the other derivatives with highter molecular weights were essentially without effect. Observations by scanning electron microscopy indicated that non-substituted, m-CH3 and o-Cl hydroxypyruvaldehyde phenylosazones caused extrusion of the erythrocyte surface, whereas o-CH3, p-CH3 and p-Cl derivatives caused invagination of the erythrocyte membrane. The p-Cl derivative can be adsorbed onto, or incorporated into the bovine erythrocyte membrane.