Effects of Deoxynivalenol on Cell Cycle and Apoptosis of Human Gastric Carcinoma Cell Line HGC-27 in vitro

Abstract

Objective To explore the putative effect of deoxynivalenol on cell cycle and apoptosis of human gastric carcinoma cell line HGC-27 in vitro.Methods HGC-27 cells were treated with DON at different concentrations(50、100、1 000、2 000μg/L) for 12,24 and 48 hours,and then cells were harvested for analysis of apoptosis,cell cycle distribution and expression of Bax,Bcl-2 and Caspase-3 with flow cytometric(FCM) and Western Blot methods.Results At relatively higher concentrations(1 000 and 2 000 g/L),DON could affect cell cycle distribution in a time dependent manner.DON treatment for 12 h,the percentages of cells in G0/G1 phases was significantly decreased and that in S phase increased.As the treatment time lasted for 24 h and 48 h,the percentages of cells in G0/G1 phases were significantly increased while that in S phase decreased(P0.05).No significant effects on G2/M phase could be found.The apoptosis rates in all DON treated groups for 12 h,24 h and 48 h were all higher than that in control.Though statistical significance in the difference of apoptosis rate between control and the DON treated groups was not found 12 h after DON treatment but it could be seen 24 and 48 h after DON treatment(P0.05).Significant dose-effect relationships were found between DON concentrations and the apoptosis rates.Western Blot analysis showed bax and caspase-3 expression was up-regulated and bcl-2 expression was down-regulated in DON treated HGC-27 cells in vitro.Conclusion DON could significantly affect the cell cycle distribution of HGC-27 cells in vitro and the effects of DON on cell cycle varied as the concentratrion and treatment time of DON changed.DON treatment could induce apoptosis of HGC-27 cell possibly by up-regulating bax and caspase-3 expression and down-regulating bcl-2 expression.