Abstract
To investigate the effects of daunorubicin (DNR) on the gene expression of brain natriuretic peptide (BNP) in myocardial cells. Myocardial cells were isolated from neonatal Wistar rats, cultured, and added with DNR of the concentrations of 0, 0.02, 0.2, and 2 mg/L respectively so as to calculate the maximum non-toxic concentration of DNR. The cultured rat myocardial cells were divided into 3 groups, DNR group, added with DNR 0.2 mg/L, DNR+fructose-1, 6-diphosphate (FDP) group, added with DNR 0.2 mg/L and FDP 2 mg/ml, and control group. The viability of the myocardial cells was examined by MTT method. RT-PCR and Western blotting were used to detect the mRNA expression and protein expression of the BNP gene. Toxicity test showed that the maximum non-toxic concentration of DNR was 0.2 mg/L. Western blotting showed that the average grey level ratio of BNP/GAPDH of the DNR group was 0.40+/-0.09, not significantly different from that of the DNR+FDP group (0.42+/-0.11, but significantly lower than that of the control group (0.82+/-0.13, P<0.01). DNR suppresses the expression of BNP gene in myocardial cells and FDP cannot reverse the suppressing effect.
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