Abstract
BackgroundThe purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells.MethodsThe levels of methylation and expression of the DACT1 gene in nasopharyngeal carcinoma tissues and CNE2 cells were determined by methylation-specific PCR and RT-PCR, respectively. CNE2 cells were treated with 5-aza-2-deoxycytidine, and the variation in the methylation status of the DACT1 gene was detected, as well as the influence of methylation on invasiveness of nasopharyngeal carcinoma cells.ResultsThe DACT1 gene was hyper-methylated in 44 of 62 cases of nasopharyngeal carcinoma. The DACT1 gene was hyper-methylated in 32 of 38 cases of nasopharyngeal carcinoma with lymph node metastasis, and the DACT1 gene was hyper-methylated in 7 of 24 cases of nasopharyngeal carcinoma without lymph node metastasis. The DACT1 mRNA level was weakly expressed or not expressed in all nasopharyngeal carcinoma tissues with hyper-methylated DACT1 genes; however, the DACT1 mRNA level was highly expressed in nasopharyngeal carcinoma tissues with low expression of the methylated DACT1 gene. The DACT1 gene was hyper-methylated and not expressed in CNE2 cells that did not have 5-aza-2-deoxycytidine treatment. After 5-aza-2-deoxycytidine treatment, the DACT1 gene was demethylated and the expression of DACT1 was restored. Moreover, the invasion ability was inhibited in CNE2 cells treated with 5-aza-2-deoxycytidine.ConclusionThe expression of DACT1 was related to the methylation status. High expression of DACT1 may inhibit the invasion and metastasis of nasopharyngeal carcinoma cells.
Highlights
The purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells
The reverse transcription polymer‐ ase chain reaction (RT-PCR) results revealed that there was no DACT1 mRNA expression in all methylated Nasopharyngeal carcinoma (NPC); DACT1 mRNA was highly expressed in unmethylated NPC tissues and the chronic inflammatory tissues in the nasopharynx (Fig. 2)
Methylation-specific PCR results showed that the DACT1 gene was hyper-methylated in CNE2 cells without 5-N-deoxycytidine treatment, but the DACT1 gene was demethylated in CNE2 cells treated with 5-azadeoxycytidine (Fig. 3)
Summary
The purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells. The occurrence and development of various tumors are related to gene methylation. Inactivation of hypermethylation in the promoter region of the tumor suppressor genes plays an important role in tumorigenesis and development of tumors. Activation of hypo-methylation of the proto-oncogene plays an important role in the development of some malignant tumors. The. Dapper1/Dpr (DACT1) gene is a tumor-associated gene that plays an important role in the regulation of tumor cell growth, proliferation, invasion, and metastasis [1]. Yang et al [2] have shown that DACT1 expression is related to pathologic grade, size, invasion, and metastasis of non-small cell lung cancers [2]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
