Effects of cyclooxygenase-2 selective inhibitor Celecoxib on the expression of P- glycoprotein in rat with status epilepticus

Abstract

Objective To study the effect of cyclooxygenase-2(COX-2)selective inhibitor Celecoxib on the expression of P- glycoprotein(P-gp)in the brain of rats with status epilepticus, in order to assess the therapeutic value of intractable epilepsy. Methods Sixty adult male SD rats were randomly divided into blank control group, the epilepsy model group and Celecoxib intervention group.The status epilepticus was induced in rats by injecting Lithium pilocarpine.Forty-eight rats were included in the experiment.There were 16 rats in each of the blank control group, epilepsy model group and Celecoxib intervention group, respectively.Immunohistochemical method and Western blot method were used to detect the expression of P-gp in experimental group in the frontal cortex and hippocampus. Results Immunohistochemistry result showed that the expression of P-gp was significantly higher in epilepsy model group than the blank control group, and the difference was statistically significant (P<0.01); The P-gp expression in the Celecoxib intervention group was lower than that in the epilepsy model group, and the difference was statistically significant (P<0.01). Western blot results suggested that the expression of P - gp could be found both in the frontal cortex and hippocampus in each group.Compared with the blank control group, the P-gp expression was significantly higher than that in the epilepsy model group, and the expression of the P-gp was lower after the Celecoxib intervention than that in the epilepsy model group, and the difference was statistically significant (P<0.05). Conclusions COX-2 inhibitor Celecoxib could decrease the expression of P-gp in brain tissue with status epilepticus, which may provide a new method for the treatment of intractable epilepsy. Key words: Cyclooxygenase-2 selective inhibitors; Status epilepticus; P-glycoprotein