Abstract
Objective: This study aimed to investigate the efficacy of curcumin (CMN) and nanocurcumin (NC) at preventing cisplatin (CDPP)-inducednephrotoxicity.Methods: Two membrane transporters, copper transporter 1 (CTR1) and organic cation transporter 2 (OCT2), have been identified involved in activeaccumulation of CDPP into renal tubular cells. We analyzed OCT2 transcription levels in rat kidney tissue and determined whether renoprotectivemechanism of CMN involves CTR1. Rats were randomly divided into five groups: (1) Control, (2) CDPP (7 mg/kg as single dose (i.p.), (3) CDPP+CMN(7 mg/kg CDPP as a single dose, i.p.+100 mg/kg/day of CMN), (4) CDPP+50 mg NC (7 mg/kg CDPP as single dose, i.p.+50 mg/kg/day NC), and(5) CDPP+100 mg NC (7 mg/kg CDPP as single dose, i.p.+100 mg/kg/day NC). Quantitative reverse transcription-polymerase chain reaction wasperformed to calculate relative expression of CTR1 and OCT2 genes in rat kidney.Results: Expression of CTR1 was unassociated with administration of CMN or NC, indicating CTR1 is uninvolved in renoprotective mechanism of CMN.The administration of 100 mg/kg/day NC increased expression of OCT2; this increase was higher compared with normal expression levels. This maybe due to another regulatory mechanism from the CMN itself.Conclusion: NC has a better renoprotective effect compared with curcumin, suggested by the increased OCT2 expression on its administration inCDPP-treated rats.
Highlights
IntroductionCisplatin (CDPP) (cis-diamminedichloroplatinum [II]) is a potent antineoplastic drug used for the treatment of solid tumors
Cisplatin (CDPP) is a potent antineoplastic drug used for the treatment of solid tumors
The median of organic cation transporter 2 (OCT2) qRT-PCR results showed an increased expression in rats treated with CDPP+100 mg NC compared with the other groups, this finding was not significant (Fig. 2)
Summary
Cisplatin (CDPP) (cis-diamminedichloroplatinum [II]) is a potent antineoplastic drug used for the treatment of solid tumors It has been extensively used for a wide variety of neoplasms including those of the lung, head and neck, breast, ovary, blood, brain, kidney, and testes [1]. The disproportionate accumulation of CDPP in kidney tissue contributes to CDPP-induced nephrotoxicity [3] It clinically manifests as a lower glomerular filtration rate, higher serum creatinine, and reduced serum magnesium and potassium levels occurring in about one-third of patients undergoing CDPP treatment [4]. This adverse effect typically evolves slowly and predictably after initial and repeated exposure [5]
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