Abstract
BackgroundCorticosterone is one of the most crucial glucocorticoids (GCs) in poultry. Our previous study shows that corticosterone can retard the longitudinal growth of bones by depressing the proliferation and differentiation of chondrocytes in broilers. The present study was designed to investigate whether corticosterone affect the development of chondrocytes and the synthesis of collagen in vitro. The chondrocytes were isolated from proximal tibial growth plates of 6-week-old broiler chickens and cultured with different doses of corticosterone for 48 h. Then the cell viability, alkaline phosphatase (ALP) activity and the expression of parathyroid hormone-related peptide (PTHrP) and type X collagen (Col X) were detected.ResultsAt 10−9-10−6 M concentration, corticosterone significantly inhibited the viability and differentiation of chondrocytes, as indicated by decreases in ALP and type X collagen expression. Conversely, there was completely opposite effect at 10−10 M. In addition, the expression of PTHrP was significantly downregulated at 10−6 M and 10−8 M, and was upregulated at 10−10 M.ConclusionsThe results suggested that corticosterone regulated chicken chondrocytes performance depending on its concentration with high concentrations inhibiting the viability and differentiation of chondrocytes and light concentrations promoting them, and these roles of corticosterone may be in part mediated through PTHrP.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-015-0398-5) contains supplementary material, which is available to authorized users.
Highlights
Corticosterone is one of the most crucial glucocorticoids (GCs) in poultry
Effect of CORT on cell viability and alkaline phosphatase (ALP) activity in cultured chicken chondrocytes The results showed that CORT inhibited chondrocytes viability and ALP activity significantly at concentrations of 10−9 M to 10−6 M in a dose-dependent manner (Figures 1 and 2)
In the present study, we investigated the effects of CORT on cultured chicken growth plate chondrocytes in terms of cell viability, ALP activity and the expression of Col X and parathyroid hormone-related peptide (PTHrP)
Summary
Our previous study shows that corticosterone can retard the longitudinal growth of bones by depressing the proliferation and differentiation of chondrocytes in broilers. The cell viability, alkaline phosphatase (ALP) activity and the expression of parathyroid hormone-related peptide (PTHrP) and type X collagen (Col X) were detected. Numerous studies had been conducted concerning the effects of GCs on growth retardation in mammals [4,5,6,7,8,9,10,11,12,13,14,15] and were to some extent controversial [16,17], whereas relatively few data were available with regard to its roles in bone development in avian. It was reported that parathyroid hormone-related peptide (PTHrP) was one of the important cytokines Both the periarticular perichondrium and growth plate
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