Effects of copper pyrithione (CuPT) on apoptosis, ROS production, and gene expression in hemocytes of white shrimp Litopenaeus vannamei

Abstract

Copper pyrithione (CuPT) is used globally to prevent biofouling. However, it poses certain risks to aquatic ecosystems. To understand the effects of CuPT on Litopenaeus vannamei after exposure to different concentrations of CuPT (0, 64, and 128 μg/L), the apoptotic cell ratio, production of reactive oxygen species (ROS), and gene expression in the hemocytes were studied at 0, 3, 12, 24, and 48 h. The results revealed that ROS production was induced significantly at 3–48 h only in the 128 μg/L groups. The apoptotic cell ratio was increased significantly at 12 and 24 h in the 64 μg/L groups, and at 3–48 h in the 128 μg/L groups. Meanwhile, CuPT exposure changed gene expression in hemocytes at different levels. In the 64 μg/L groups, the expression of Mn-superoxide dismutase (MnSOD) was induced at 12 h, glutathione peroxidase (GPx) was induced at 24 and 48 h, caspase-3 induced at 24 h, metallothionein (MT) and HSP70 were increased at 3 h. In the 128 μg/L groups, MnSOD was increased at 3 h and then decreased at 12–48 h, GPx was up-regulated at 3, 24 h and then decreased at 48 h, caspase-3 was increased at 24 h, MT was increased at 3–48 h, HSP60 and HSP70 were up-regulated at 3–12 h. These results indicated that CuPT induced ROS production and the expression of caspase-3 in hemocytes, then caused hemocyte apoptosis. Expression levels of MnSOD, GPx, MT, HSP60, and HSP70 were up-regulated to protect the hemocyte against CuPT stress.