Abstract
The aim of this study was to detect the effects of different cooling rates and different ice-seeding temperatures on the cryopreservation of whole ovaries. Cow whole ovaries were slowly frozen using different protocols with different cooling rates and different ice-seeding temperatures. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the follicular densities of grafts were measured. Protocol IIb was most effective protocol. Protocol Ib was more effective than protocol Ia and protocol Ic, and protocol IIIb was more effective than protocol IIIa and protocol IIIc. Protocol IIb (the cooling rate was 0.2°C/min, and the ice-seeding temperature was -5°C) was appropriate for slow freezing of cow whole ovaries.
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