Abstract
The static magnetic field was shown to affect the proliferation, adhesion and differentiation of various types of cells, making it a helpful tool for regenerative medicine, though the mechanism of its impact on cells is not completely understood. In this work, we have designed and tested a magnetic system consisting of an equidistant set of the similar commercial permanent magnets (6 × 4 assay) in order to get insight on the potential of its experimental usage in the biological studies with cells culturing in a magnetic field. Human dermal fibroblasts, which are widely applied in regenerative medicine, were used for the comparative study of their proliferation rate on tissue culture polystyrene (TCPS) and on the polyacrylamide ferrogels with 0.00, 0.63 and 1.19 wt % concentrations of γ-Fe2O3 magnetic nanoparticles obtained by the well-established technique of laser target evaporation. We used either the same batch as in previously performed but different biological experiments or the same fabrication conditions for fabrication of the nanoparticles. This adds special value to the understanding of the mechanisms of nanoparticles contributions to the processes occurring in the living systems in their presence. The magnetic field increased human dermal fibroblast cell proliferation rate on TCPS, but, at the same time, it suppressed the growth of fibroblasts on blank gel and on polyacrylamide ferrogels. However, the proliferation rate of cells on ferrogels positively correlated with the concentration of nanoparticles. Such a dependence was observed both for cell proliferation without the application of the magnetic field and under the exposure to the constant magnetic field.
Highlights
Regenerative medicine focuses on the curing and replacement of whole organs or tissues damaged as a consequence of trauma, disease, age or innate defects across a wide number of dermal specific types of treatments [1,2]
Polyacrylamide hydrogels with different cross-linking density of the polymer network and polyacrylamide-based ferrogel (FG) with embedded magnetic nanoparticles (MNPs) of the iron oxide obtained by the laser target evaporation technique (LTE) [7] were synthesized and studied
The obtained results were related to the heating capacity of nanoparticles [10,11], influence of the presence of LTE MNPs on the cell morphology [12], adhesion and proliferation, and this research direction seems to be specially promising for cellular technologies and tissue engineering applications
Summary
Regenerative medicine focuses on the curing and replacement of whole organs or tissues damaged as a consequence of trauma, disease, age or innate defects across a wide number of dermal specific types of treatments [1,2]. We have proposed a new approach for cultivation of selected cell cultures, which might be useful for regenerative medicine, drug delivery and biosensor applications. This approach is based on the usage of synthetic hydrogels and ferrogels as biomimetic materials [3,4,5]. The obtained results were related to the heating capacity of nanoparticles [10,11], influence of the presence of LTE MNPs on the cell morphology [12], adhesion and proliferation, and this research direction seems to be specially promising for cellular technologies and tissue engineering applications
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