Abstract
Conjugated linoleic acids (CLAs) play a major role in adipocyte differentiation and lipid metabolism in animals. MicroRNAs (miRNAs) appear to be involved in many biological processes in adipose tissue. However, the specific influence on miRNAs by CLA supplementation in porcine adipose tissue remains unclear. Thus, we continuously added 1.5% CLA to the pig diet from the embryo stage to the finishing period and conducted a high-throughput sequencing approach to analyse the changes in adipose tissue miRNAs. We identified 283 known porcine miRNAs, and 14 miRNAs were differentially expressed in response to CLA treatment. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the targets of the 14 differentially expressed miRNAs were involved in the Wnt signalling pathway. The CLA treatment downregulated the gene expression of PPARγ, C/EBPα, FAS, and FATP1 in both subcutaneous and abdominal fat tissues; the analysis showed that ssc-miR-21 expression was significantly correlated with PPARγ expression (p < 0.05), and speculated that ssc-miR-21 might influence adipogenesis through PPARγ. In conclusion, our study analysed the miRNA profiles in porcine adipose tissues by CLA treatment, and demonstrated that miRNAs are important regulators of fat lipogenesis. This study provides valuable information for the molecular regulatory mechanism of CLA on adipose tissue.
Highlights
Conjugated linoleic acid (CLA) is an octadecadienoic acid containing a conjugated double bond and is often found in fats from ruminants
MicroRNAs are a class of small, non-coding RNAs that modulate gene expression at the post-transcriptional level in animals and plants. They are involved in various biological processes, including cell growth, differentiation, apoptosis, and metabolism [10,11,12]. miRNAs can influence the metabolism of adipose tissue, and Meng et al reported that metabolic syndrome (MetS) changed the miRNA expression profile of porcine adipose tissue mesenchymal stem cell (MSCs)-derived extracellular vesicles (EVs), and that 14 and eight distinct miRNAs were enriched in
Thisten study, we examined the effect at three growth stages in subcutaneous adipose tissues, while six miRNAs were differentially of CLA treatment on the miRNome expression in swine adipose tissue by using high-throughput expressed in abdominal adipose tissues at 240 days old
Summary
Conjugated linoleic acid (CLA) is an octadecadienoic acid containing a conjugated double bond and is often found in fats from ruminants. Several studies have shown that CLA plays an important role in reducing adipose fat in animals [5]. MicroRNAs (miRNAs) are a class of small, non-coding RNAs that modulate gene expression at the post-transcriptional level in animals and plants. They are involved in various biological processes, including cell growth, differentiation, apoptosis, and metabolism [10,11,12]. CLA was the most appropriate dose for improving the carcass traits and meat quality of pigs, and CLA significantly altered the expression of miR-27, miR-143, and adipocyte differentiation genes in adipose tissue of growing pigs [21]. MiRNAs following 1.5% CLA supplementation from the embryo stage to the finishing period and explored which miRNAs were implicated in the process with the aim of elucidating the molecular mechanisms of CLA on adipose development
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