Effects of conjugated linoleic acid on growth and cytokine expression in Jurkat T cells

Abstract

Conjugated linoleic acid (CLA) has shown beneficial properties in animal models including anti-cancer, anti-atherogenic and anti-diabetic effects, while contrasting immunological effects were reported. While its anti-inflammatory activity has been associated to inhibition of arachidonic acid biosynthesis and to peroxisome proliferator-activated receptors (PPARs) activity, the molecular pathways underlying its immunoenhancing activity are essentially unknown. The aim of our study was to examine whether CLA showed specific effects in vitro on a T cell model, represented by the Jurkat cell line. CLA was found non toxic for Jurkat in the range 50–200μM, as assessed by LDH release; however, incubation with 50μM CLA was associated to a significant inhibitory effect on cell proliferation. The analysis of IL-2 and IFN-γ transcript levels, produced in stimulated Jurkat cells, showed an increased expression of both cytokines in CLA-treated cells. Interestingly, the increased induction of IL-2 but not of IFN-γ mRNA, could be suppressed by co-incubation with Gö 6976, a protein kinase C (PKC) inhibitor. Co-incubation with superoxide dismutase (SOD) or N-acetyl-l-cysteine (NAC) restored the basal levels of RNA synthesis for both cytokines. Taken together, these results suggest a specific role for dietary CLA in the modulation of the immune response in a T cell line model that is mediated, at least in part, by PKC and through the production of oxidative molecules.