Abstract
We investigated the effect of glycine and other osmotic effectors on DNA and chromatin precipitation by mono-, di- and multivalent cations and histone H1. The addition of these compounds drastically reduces the precipitation effects with an efficiency in the order taurine > glycine > proline and sorbitol > inositol > betaine. Aminocarboxylic acids with increasing distance between the charged C- and N-terminal groups displayed enhanced efficiency in the protection effect against DNA precipitation. We interpreted these observations on the basis of Manning's counterion condensation theory, taking into account the increase in dielectric constant upon osmotic effector addition. 23Na-NMR was used to evidence sodium counterions release as a result of this increase in dielectric constant.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
