Abstract
The combustion explosion of ordinary diesel is a global environmental problem. Green microalgae, which do not cause eutrophication, are a raw material that can be used to clean biodiesel. To increase lipid productivity, this study used a nitrogen deficient & mixotrophic (+Glucose) culture of lipid-producing microalgae Parachlorella kessleri TY from Shanxi Province, China. To examine the growth of P. kessleri TY, we measured dry weight, chlorophyll content, and chlorophyll fluorescence intensity under different culture conditions, in addition to the contents of neutral lipids, total lipids, and fatty acids, to examine its lipid accumulation ability. Cells were cultured in autotrophic, nitrogen deficient, mixotrophic (+Glucose), and nitrogen deficient & mixotrophic (+Glucose) conditions for 7 days. We found the growth of P. kessleri TY under nitrogen deficient & mixotrophic conditions was higher than that under the autotrophic and nitrogen deficient conditions, but lower than that under the mixotrophic (+Glucose) conditions. However, its lipid accumulation ability was significantly higher than that of control cultures. In conclusion, P. kessleri TY cultured under nitrogen-deficient and mixotrophic (+Glucose) conditions has significant lipid production capacity. Our results provide a theoretical basis for the use of microalgae as a raw material in the production of biodiesel, and promote the application of P. kessleri TY in large-scale production.
Highlights
As a result of socio-economic growth, the global demand for energy has multiplied, and the world’s staggering energy consumption has prompted researchers to search for new renewable and environmentally friendly fuels
When the nitrogen deficient and the mixotrophic (+Glucose) condition acted on the algal cells at the same Water 2021, 13, x FOR PEER REVIEWtime, the dry weight and biomass productivity were affected by the nitrogen element and the carbon source
Juntila et al [37] examined the growth and lipid accumulation of Chlorella sorokiniana in medium supplemented with glucose under nitrogen deficient conditions, and demonstrated that C. sorokiniana was under the nitrogen deficient & mixotrophic (+Glucose) culture, the biomass productivity and lipid productivity were lower than those under the normal nitrogen & mixotrophic (+Glucose) culture
Summary
As a result of socio-economic growth, the global demand for energy has multiplied, and the world’s staggering energy consumption has prompted researchers to search for new renewable and environmentally friendly fuels. The components of ordinary diesel engine emission pollutants are complex and include some unconventional pollutants that have significant impacts on the atmospheric environment and human health, such as polycyclic aromatic hydrocarbons (PAHs) and oxygen-containing hydrocarbons. Biodiesel is considered a clean fuel, and the pollutant emission characteristics of biodiesel are different from those of ordinary diesel. Their impacts on human health are different. It was reported that biodiesel with rapeseed oil and soybean oil as raw materials reduces PAH emission from diesel engines [2]. The use of biodiesel can reduce the harm of diesel engine emissions to the atmospheric environment and human health
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