Effects of Combination Therapy with Cisplatin and Grape Seed Proanthocyanidins on Human Cervical Cancer Cells C33A

Abstract

To investigated the inhibitory effect of cisplatin in combination with Grape Seed Proanthocyanidins against human cervical cancer cell lines in vitro. Cervical cancer C33A cells were cultured and divided into four groups: cisplatin group, Grape Seed Proanthocyanidins group, cisplatin with Grape Seed Proanthocyanidins group and the blank control group. Morphologic changes of C33A cells were observed under invert microscope after C33A cells were treated 48, and 72 h. The proliferation of C33A cells was determined by CCK8 assay. Apoptosis were evaluated by PE Annexin V staining. Both of these two agents induced apoptosis and inhibited proliferation in C33A cells in a dose-dependent manner. In combination, cisplatin and Grape Seed Proanthocyanidins amplified these regulatory effects compared to each agent alone. The inhibition of proliferation can be observed in cisplatin group and in Grape Seed Proanthocyanidins group with different concentrations, while the notable effect was showed in Grape Seed Proanthocyanidins group. Combination treatment of cisplatin and Grape Seed Proanthocyanidins produces synergistic influence on C33A cells.