Effects of cold stress, alprazolam and phytomedicine in combination with stress on blood glucose and haematogical parameter of the male albino rat

Abstract

The present study conducted to investigate the haematological changes and changes of blood glucose level in male albino rat due to cold stress. In this experiment normal 12:12 light dark phases were maintained for all the groups. Control group was kept at normal room temperature (22 ± 1). A (4°C), B in (0°C), C (4°C and 0.30 mg alprazolam / kg body weight /animal), D (0°C and 0.30 mg alprazolam/ kg body weight/ animal. E2 group was treated with (4°C and 1000 mg/kg body weight methanolic extract of Withania somnifera root extract /animal). F2 group was treated with (0°C and 1000 mg/kg body weight methanolic root extract of Withania somnifera / animal). The blood glucose level was significantly increased in stressed rats compared to the control animals. The results were also consistent with the exposure to the stress and chronic restraint stress. Action of Alprazolam over cold stress treated group significantly reduced the blood glucose level. Whereas methanolic root extract of Withania somnifera in low and high doses also showed significant effects to the control anxiety like effects on blood glucose level. Alprazolam + different stress treated groups in different experiment at conditions show significant changes in its haematological parameters in comparison to the stress treated group. Whereas herbal medicine (i.e., methanolic root extract of Withania somnifera) when applied to different stress treated group showed more significant result, compared to the Alprazolam+ different stress treated groups. The positive safe anti stress effects of the herbal plant medicine prove that the tribal medicines have the potentiality to act effectively and can be used as safe medicine for antistress purposes.