Effects of cold atmospheric pressure plasma jet on human apical papilla cell proliferation, mineralization, and attachment.

Abstract

To investigate the direct effects of cold atmospheric pressure plasma jet on cell proliferation and mineralization on human apical papilla cells and its indirect effect on cell attachment on plasma-treated dentin. Plasma was directly applied to cell culture for various durations. Cell proliferation was evaluated using AlamarBlue® assay. Mineralization was assessed using Alizarin Red S staining after 14 and 21 days. The cell attachment to plasma-treated dentin surface was evaluated using an ex vivo immature tooth model, and the protocols varied based on root canal irrigants (NSS or 17% EDTA), the durations of plasma application (0, 30, or 60 s), and the different positions of the plasma needle (coronal or middle). The attached cells were visualized using the immunofluorescence staining and the positive-staining cells were counted. There was no difference in the cell proliferation between the untreated and plasma-treated cells. However, the plasma-treated cells tended to have lower levels of calcium deposition, especially after the 60-s plasma application (p < 0.05). Finally, significantly greater numbers of attached cells were shown when NSS was combined with plasma treatment when compared to the untreated cells (p < 0.05), whereas no difference was observed when EDTA was used as the irrigant. Plasma might disturb the mineralization of the cells. Interestingly, the dentin conditioning process using a plasma jet with NSS irrigation may enhance cell attachment. Cold atmospheric pressure plasma jet may be an alternative treatment in regenerative endodontic procedures in order to improve cell attachment in the root canal system.