Abstract
Background:Ischemia-reperfusion (I/R) induced by testicular torsion can damage the testicles. In the present study, we assessed the effects of coenzyme Q10 (CoQ10) and diamond nanoparticles on sperm parameters in I/R testes in rats.Materials and Methods:Forty-eight Wistar adult male rats were divided into eight groups: healthy control (Ch), diamond nanoparticle healthy control group (Ch+Dia), CoQ10 healthy control group (Ch+Q10), diamond nanoparticles+CoQ10 healthy control group (Ch+Q10+Dia), torsion/detorsion group (Ct), the Ct group that received diamond nanoparticles (Ct+Dia), the Ct group that received CoQ10 (Ct+Q10), and Ct group that received diamond nanoparticles and CoQ10 (Ct+Q10+Dia). The rats were euthanized, and we collected the semen from the epididymal tissues to evaluate sperm viability, motility, concentration, and morphology parameters.Results:The I/R of the testicles significantly reduced sperm concentration, motility, viability, and altered sperm morphology in the rats. However, the administration of CoQ10 significantly improved sperm parameters in the rats with testicular I/R. Diamond nanoparticles decreased the sperm parameters; however, simultaneous administration of diamond nanoparticles and CoQ10 led to improved sperm parameters. Conclusion:CoQ10 potentially appeared to have protective effects against the long-term side-effects of I/R in testes in rats. Co-administration of diamond nanoparticles with CoQ10 significantly improved sperm parameters and greatly reduced the negative effects of diamond nanoparticles alone. Therefore, green synthesis of nanoparticles with the use of antioxidants such as CoQ10 is recommended.
Highlights
Sperm production occurs inside the seminiferous tubules, after which sperm are transferred to the epididymides for storage
We examined the effect of diamond nanoparticles on testicular ischemia and noted a decreased effect of diamond nanoparticles on the rate of viable sperm
Sperm viability was lower in rats with ischemic testicles that received the diamond nanoparticles compared with the group with ischemic testicles that received coenzyme Q10 (CoQ10)
Summary
Sperm production occurs inside the seminiferous tubules, after which sperm are transferred to the epididymides for storage. Studies show that antioxidant compounds and free radical scavengers have protective effects against the damage caused by the I/R process [7]. Due to the significant effect of free radicals in causing I/R lesions, the combination of CoQ10 with diamond nanoparticles appears to be effective in reducing both inflammatory precursors and I/R damage to the testicles. The present study aimed to investigate the effect of CoQ10, as an antioxidant, and diamond nanoparticles on testicular function in rats with I/R damages. We assessed the effects of coenzyme Q10 (CoQ10) and diamond nanoparticles on sperm parameters in I/R testes in rats. The administration of CoQ10 significantly improved sperm parameters in the rats with testicular I/R. Co-administration of diamond nanoparticles with CoQ10 significantly improved sperm parameters and greatly reduced the negative effects of diamond nanoparticles alone. Green synthesis of nanoparticles with the use of antioxidants such as CoQ10 is recommended. [GMJ.2021;10:e2029] DOI:10.31661/gmj.v10i0.2029
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