Effects of coagulation factors and inflammatory cytokines on development of acute myocardial infarction in patients younger than 60 years

Jun-Jie Wang,Mei-Yun Fang

doi:10.4314/tjpr.v15i4.23

Abstract

Purpose : To investigate the effects of coagulation factors and inflammatory cytokines on acute myocardial infarction (AMI) development in patients younger than 60 years. Methods : In this study, 60 patients admitted to The First Affiliated Hospital of Dalian Medical University (Dalian, China) with AMI and 30 other subjects matched with the patients for age and ethnicity but without AMI were enrolled. Blood samples were collected from the AMI patients and the control subjects after a 12-h fast. Subsequently, the levels of coagulation factors (F) II (FII), VII (FVII), VIII (FVIII), fibrinogen (Fg) and von Willebrand factor (vWF) in plasma were analyzed by enzyme-linked immunosorbent assay (ELISA). The protein expression levels of these coagulation factors were determined by Western blot analysis. Inflammatory factors including C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were also measured by ELISA. Results : FII, FVII, FVIII, Fg and vWF levels in plasm were significantly higher in AMI patients compared with control subjects (p 0.05). However, TNF-α and IL-6 levels in the plasma of AMI patients were significantly higher than those in control subjects (p < 0.05). Conclusion : The results reveal that the pathogenesis of AMI in patients younger than 60 years might be closely related to the high levels of coagulation factors and inflammatory cytokines in the blood. Keywords : Coagulation factor, Inflammatory cytokines, Acute myocardial infarction, C-reactive protein, Tumor necrosis factor-α, Interleukin- 6

Highlights

Coronary heart disease (CHD) has become one of the leading global threats to human health [1]
The present study was aimed to investigate the effects of coagulation factors and inflammatory cytokines on the development of acute myocardial infarction in patients younger than 60 years, findings that would be beneficial for the accurate diagnosis and effective treatment of Acute myocardial infarction (AMI) in the clinic
Our results showed that there were no significant differences in the release of C-reactive protein (CRP) between AMI patients and the control subjects (p > 0.05)

Summary

INTRODUCTION

Coronary heart disease (CHD) has become one of the leading global threats to human health [1]. The systemic inflammation response has been documented in patients with AMI, and includes the elevation of circulating inflammatory cytokines such as C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), and interleukin (IL-6) [9,10]. The present study was aimed to investigate the effects of coagulation factors and inflammatory cytokines on the development of acute myocardial infarction in patients younger than 60 years, findings that would be beneficial for the accurate diagnosis and effective treatment of AMI in the clinic. Sixty patients (39 males and 21 females) admitted to The First Affiliated Hospital of Dalian Medical University (Dalian, China) with a diagnosis of AMI, defined according to the guidelines of the WHO MONICA protocol [11], were studied. Coagulation factors including FII, FVII, VIII (FVIII), Fg and vWF in the plasma were analyzed by enzyme linked immunosorbent assay (ELISA) according to the manufacturer’s instructions.

RESULTS

DISCUSSION

CONCLUSION

11. WHO MONICA Project