Effects of Co-factor and Deoxycytidine Substituted Oligonucleotides upon Sequence-specific Interactions between MspI DNA Methyltransferase and DNA

Abstract

MspI methyltransferase (M. MspI) catalyses the transfer of a methyl group from S-adenosyl- l-methionine to the C-5 position of the outer deoxycytidine base in the DNA sequence 5′-CCGG-3′. Recombinant M. MspI when expressed and purified as a translational fusion with glutathione-S-transferase, shows all of the properties of the wild-type enzyme. We report the kinetic analysis of M. MspI binding to DNA, which suggests a two-stage methylation process, whose initial DNA binding rate is governed by the presence of a positively charge sulphonium centre on the cofactor. Results are also presented that indicate that M. MspI binds preferentially to hemi-methylated DNA and that full methylation of either deoxycytidine on both strands significantly impairs sequence-specific protein-DNA interactions. Furthermore, the importance of the 4-amino group of the inner deoxycytidine for sequence-specific protein-DNA interactions is demonstrated by substituting deoxycytidine with 2-pyrimidinone-1-β- d-2-deoxyriboside. In addition, we detail the intrinsic structural elements of a cofactor, required to enhance the binding of M. MspI to its recognition sequence, by using S-adenosyl- l-methionine and a range of derivatives.