Effects of clozapine and its metabolites on the 5-HT 2 receptor system in cortical and hippocampal cells in vitro

Abstract

Purpose: The goal of the present study was to determine the effects of clozapine (Cloz) and its metabolites norclozapine (Norcloz) and clozapine-N-oxide (Cloz-N-oxide) on the 5-HT 2 receptor system on the levels of protein and gene expression in in vitro systems of primary cortical cells of the rat and human hippocampal SHS5Y5 neuroblastoma cells. Methods: Clinically relevant concentrations of Cloz (200/400 ng/ml) and its metabolites (200 ng/ml) were used for the examination of the effects of Cloz and its metabolites on serotoninergic 5-HT 2 receptor parameters (density, affinity and mRNA levels) as well as on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels in primary cortical cells of the rat after treatment for 24 h under in vitro conditions. To compare the results to human cells, we also measured treatment-induced changes in 5-HT 2 and GAPDH mRNA levels in human hippocampal SHS5Y5 cells. Results: A significant decrease was found in primary cortical cells for 5-HT 2 receptor density (Cloz 200/Cloz 400/Norcloz 200 and Cloz-N-oxide 200 vs. control) and 5-HT 2A receptor mRNA levels (Cloz 200 vs. control). 5-HT 2A receptor mRNA levels were also significantly reduced (Norcloz 200 vs. control) in SHS5Y5 cells. GAPDH mRNA levels were not affected. Conclusions: The results of the present study show that Cloz and Norcloz induce significant alterations on the 5-HT 2 receptor system in primary cortical cells of the rat and in human hippocampal cells.