Effects of Cisplatin and Pemetrexed on the cell viability and cell adhesion of benign mesothelial and mesothelioma cells

Abstract

Introduction: Cisplatin (C) and Pemetrexed (P) are first-line chemotherapy treatment for patients with non-resectable malignant pleural mesothelioma (MPM). Their effects are unknown on cell phenotypes pertinent to the MPM patho-biology such as cell viability and cell adhesion on homologous cell derived extracellular matrix (ECM). Aim: To evaluate the effects of C, P and their combination (C+P) on cell viability and cell adhesion. Methods: MeT-5A(benign mesothelial cells), M14K(epithelioid), MSTO (biphasic) and ZL34(sarcomatoid) were used. Drug effects (C:10−5 M; P:2x10−4 M) on cell viability at 48hrs and cell adhesion at 90mins were tested on ECM. Crystal violet staining and elution was used to measure cells. 10% RPMI was used as Control(Con). Results: Cell viability was significantly lower in all cell lines treated with C, P or C+P (MeT-5A: Con 100±2.7%, C 91.9±3.2%, p Conclusions: Cell viability was significantly lower in all cell lines with C, P or C+P. Cell adhesion was significantly lower in MSTO and ZL34 on treatment with C, P or C+P. In M14K only C+P significantly lowers cell adhesion.