Effects of ciprofloxacin on the enteritis of interleukin-10 knockout mice after ileocaecal resection

Abstract

Objective To investigate the effects of ciprofloxacin on anastomotic stoma,small intestinal inflammation and fibrosis in interleukin-10 knock-out mice （mice model of Crohn′s disease （CD）） after ileocaecal resection.Methods Twelve interleukin-10 knockout male C3H/HeJBir mice were evenly divided into control group and ciprofloxacin group.The mice model was established with ileocaecal resection and ileocolostomy.There was no intervention in control group.Ciprofloxacin （50 mg/kg per day）was orally administrated in ciprofloxacin group for four weeks.All the mice were executed.The intestine around anastomotic stoma was taken for hematoxylin-eosin （HE）and Sirius red staining and histological scoring.The level of interferon （IFN）-γand tumor growth factor （TGF）-βin intestine around anastomotic stoma and small intestine was detected by enzyme-linked immunosorbent assay （ELISA） method.The content of procollagen α1 was determined by real-time polymerase chain reaction.The data were analyzed by t test.Results The inflammatory reaction level of anastomotic stoma and small intestine in mice of ciprofloxacin group （2.50±0.55 and 1 .67±0.52）was significantly lower than those of control group （3.67±0.63 and 2.83±0.75 ,t=-8.273,-5 .188,both P 〈0.05 ）.The IFN-γof anastomotic stoma and small intestine also significantly decreased （（0.72 ±0.22 ）pg/mg vs （1 .76 ±0.43 ）pg/mg, （0.23±0.12）pg/mg vs （0.78 ± 0.52 ）pg/mg;t = - 7.354,- 5 .268,both P 〈 0.05）.However, ciprofloxacin could not significantly decrease fibrosis in anastomotic stoma and small intestine of CD mice. There was no significant difference in the level of TGF-βcompared with control group.Conclusions The role of ciprofloxacin in improving inflammatory reation in anastomotic stoma of CD mice was limited. Maintenance therapy may be necessary to reduce the relapse of anastomotic stoma diseases. Key words: Ciprofloxacin; Inflammatory bowel diseases; Mice