Abstract
This data article describes the influence of Cimicifuga racemosa extract Ze 450 on neuronal cells in models of glutamate-induced excitotoxicity and cell death induced by oxidative stress. Effects of Ze 450 on glutamate-mediated excitotoxicity were assessed in primary cortical rat and mouse neurons and, further, glutamate-mediated oxidative stress was analyzed in HT22 cells lacking ionotropic glutamate receptors. This study especially focusses on mitochondrial parameters like mitochondrial ROS formation, mitochondrial membrane potential, ATP production and mitochondrial integrity. Further the effects of Ze 450 on lipid-peroxidation, metabolic activity, cell proliferation and cell death were assessed under control conditions and oxidative challenge evoked by millimolar concentrations of glutamate in HT22 cells. These data support the findings in HT22, mHypo and HepG2 liver cells (Rabenau et al., 2018) [1].
Highlights
This data article describes the influence of Cimicifuga racemosa extract Ze 450 on neuronal cells in models of glutamate-induced excitotoxicity and cell death induced by oxidative stress
Cell death was analyzed using Annexin V/PI FACS staining, metabolic activity was determined via MTT assay, cell proliferation was measured with the xCELLigence system, mitochondrial integrity was analyzed via MitoTRACKER red staining and following fluorescence microscopy and lipid-peroxidation (BODIPY), mitochondrial membrane potential (TMRE) and mitochondrial ROS formation (MitoSOX) were determined via FACS analysis
Metabolic switch induced by Cimicifuga racemosa extract prevents mitochondrial damage and oxidative cell death, DOI:10.1016
Summary
HT22 cells (kindly provided by David Schubert, Cellular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA) were grown in Dulbecco's modified Eagle medium (DMEM, Capricorn Scientific GmbH, Germany) supplemented with 10% heat-inactivated fetal calf serum (Merck, KGaA, Germany), 100 U/ml penicillin, 100 mg/ml streptomycin (Merck KGaA, Germany)and 2 mM glutamine (Merck KGaA, Germany). 3–10 mM glutamate (Merck KGaA, Germany) was added to the medium for the indicated amount of time (8–16 h). Cultures were grown in neurobasal medium (ThermoFisher Scientific, Germany) supplemented with 1.2 mM glutamine, 2% B27 supplement (Invitrogen, USA), 100 U/ml penicillin and 100 mg/ml streptomycin. Marburg University, Germany) were plated on PEI coated 96 well plates with 20,000 cells/well and handled to the mouse neurons
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