Effects of Chrysin on intestinal inflammation and gut microbiota in lipopolysaccharides‐induced mice

Abstract

Chrysin is a natural flavonoid with a variety of biological activities such as antioxidant, antivirus, antitumor, antidiabetic and so on. However, the potential protective effects of chrysin, against lipopolysaccharides (LPS)‐induced intestinal inflammation and its associated alteration of gut microbiota have not been studied. In the present study, male C57BL6/J mice were randomly divided into three groups and fed either normal chow diet (CON and LPS groups) or that supplemented with 1% (wt/wt) chrysin (CHY group) for 28 days followed by a single intraperitoneal injection of LPS (10 mg/kg BW). Control mice were given normal saline and all animals were kept for 72 hours before sacrifice. The results showed that chrysin attenuated histological injury of intestine and the production of pro‐inflammatory cytokines, TNFα, IL‐6 and IL‐1β. When compared to the LPS group revealed at genus level, CHY increased the proportion of Alloprevotella and Lachnospiraceae NK4A136, and decreased Escherichia_Shigella and Akkermansia. Furthermore, LPS group showed lower species richness of gut microbiota compared to the control animals, and the reduction was reversed by the addition of chrysin. In conclusion, chrysin can attenuate the LPS‐induced intestinal damage in mice. This protective effect appears to be mediated, in part, through beneficial changes to the microbiota.Support or Funding InformationThis study was supported by the National Science Council Grant 105‐2320‐B‐468‐001 Taipei, Taiwan.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.