Effects of chronic copper exposure on fluvial systems: Linking structural and physiological changes of fluvial biofilms with the in-stream copper retention

Abstract

Long-term metal exposure is known to be responsible for a large variety of structural and functional changes in periphyton communities which allow these communities to adapt to metal-polluted conditions. This study aimed to link the changes that chronic copper (Cu) exposure causes on the structure and physiology of fluvial biofilms with the efficiency of the river systems in retaining phosphate and Cu. The effects of a chronic Cu exposure on the structure, physiology and induction of Cu tolerance of the community were evaluated by comparing this community with a non-exposed one. Results showed that periphyton chronically exposed to Cu had lower algal biomass, higher proportion of green algae, lower proportion of brown algae, and higher EPS content per unit of biomass than the un-exposed community. In addition, the chronically-exposed community showed a Cu content (both total and intracellular Cu content) ten times higher than the un-exposed community. While in-stream phosphate retention was not markedly influenced by chronic Cu exposure; Cu retention was clearly reduced, as was shown by a reduction in Cu retention efficiency (Cu– S w) and demand (Cu–Vf). The chronically-exposed periphyton, in spite of having high intracellular Cu concentration, showed similar photosynthetic efficiency than the un-exposed community and showed a higher Cu tolerance. It indicated that this community was acclimatized to Cu exposure and that this acclimatization was probably linked to the ability to detoxify and immobilize metals. These observations suggest that the fate of Cu in fluvial ecosystems will be influenced by the exposure history of the system. The results from this study indicate that metals will travel longer distances in metal-polluted streams compared to pristine systems having effects on water quality farther downstream.