Effects of chronic cadmium exposure on metamorphosis, skeletal development, and thyroid endocrine disruption in Chinese toad Bufo gargarizans tadpoles

Abstract

The present study examined the effects of chronic cadmium (Cd) exposure on metamorphosis, body size, thyroid gland, and skeletal development of Chinese toad (Bufo gargarizans) tadpoles. Tadpoles were exposed to Cd concentrations at 0, 5, 10, 50, 100, and 500 μg/L from Gosner stages 26 to 46 of completion of metamorphosis. Our results showed that 100 and 500 μg/L of Cd concentrations increased mortality and decelerated metamorphosis rate. In addition, significant body size reduction at Gosner stage 42 was observed at 100 and 500 μg/L of Cd treatments (p < 0.01). Average body length and hind-limb length were significantly decreased in the 500 μg/L of Cd group (p < 0.05) but body mass was not significantly different at Gosner stage 46. Moreover, bone formation was delayed in high Cd concentration treatments (50, 100, and 500 μg/L) at both Gosner stage 42 and 46. Histopathological changes of the thyroid gland showed that follicular cell hyperplasia and malformation were induced by high Cd concentrations (50, 100, and 500 μg/L). Furthermore, real-time polymerase chain reaction analysis suggested that the larvae exposed to high-dose Cd exhibited a significant decrease in deiodinase (Dio2) and thyroid hormone receptor (TRβ) mRNA levels at Gosner stage 42 and 46. Our investigation indicated that high-dose Cd caused metamorphic deceleration, body size reduction, and delayed skeletal development through disrupting the thyroid system in B. gargarizans larvae. Environ Toxicol Chem 2018;37:213-223. © 2017 SETAC.