Abstract
Background: Poly‐adenosine diphosphate (ADP)‐ribosylation is involved in a variety of biological processes, which include DNA repair, malignant transformation, and apoptosis. It is of interest how this reaction is altered after long‐term alcohol intake. Therefore, we determined long‐term alcohol effects on hepatic poly‐ADP‐ribosylation in the rat.Methods: Male Sprague Dawley® rats (four pairs) were pair‐fed a nutritionally adequate liquid diet that contained ethanol as 36% of total energy and an isocaloric control diets for 4 weeks. Liver tissue homogenates and nuclear fractions were subjected to ADP‐ribosylation with [32P]nicotinamide adenine dinucleotide. The ADP‐ribosylated proteins were separated by SDS‐PAGE, followed by autoradiography. Expression of poly‐ADP‐ribose polymerase (PARP) also was evaluated by Western blotting.Results: Incubation of rat liver homogenates in ADP‐ribosylation reaction mixture resulted in a radiolabeling of a 116 kDa protein, most likely auto‐ribosylation of PARP. This poly‐ADP‐ribosylation was increased significantly (p < 0.025) after long‐term alcohol intake. This alcohol effect was reproducible in nuclear fractions as well. Expression levels of PARP, however, were comparable between alcohol‐fed rats and their pair‐fed controls.Conclusion: Poly‐ADP‐ribosylation, an important posttranslational modification of nuclear proteins, was increased significantly after chronic alcohol consumption in the rat.
Published Version
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