Effects of chronic administration and withdrawal of phlorotannin supplement on sleep-wake profiles in mice: A comparative study with the hypnotic drug diazepam

Abstract

The aim of this study was to investigate the effects of chronic administration (21 days) of phlorotannin supplement (PS) on sleep and compare them with the effects of diazepam (DZP), a benzodiazepine hypnotic drug. DZP significantly decreased sleep latency and increased non-rapid eye movement sleep amount on day 1 of administration compared with those at baseline; however, its effects diminished over the administration period. On the contrary, PS showed significant sleep-promoting effects throughout the study period, with no significant difference in efficacy between days 1 and 21. Notably, PS did not cause any changes in the slow wave activity (SWA) throughout the treatment period; whereas, DZP decreased the SWA on day 1 of administration. These results suggest that chronic administration of PS does not induce tolerance, implying its consistent sleep-maintaining effects.