Abstract
This experiment was designed to examine the effects of choline and inositol in Eagle's minimal essential medium (MEM), because the values of specific binding were increased when the 3H-dihydroalprenolol (3H-DHA) binding assay of β-adrenoceptors in the guinea pig and rat cerebral cortical membranes was carried out in Eagle's MEM instead of 60 mM Tris-HCl, 20 mM MgCl2 buffer (pH 7.4). All chemicals contained in the MEM were added to the 3H-DHA binding mixture and the values of the specific binding were compared with the control values obtained in the absence of the chemicals. The presence of choline (0.1-10 μM) or inositol (0.1-10 μM) induced an increase of the values of the specific binding. These effects of choline and inositol were observed after preinclubation for 20 and 1 min, respectively. After 30 min of incubation, no changes were observed upon removal of these chemicals. Furthermore, choline and inositol significantly increased the β-adrenoceptor density (β ) when 3H-DHA and I-iodocyanopindolol were used as the radioligands, as determined by Scatchard analysis, but no significant change was observed in the value of the dissociation constant (Kd) in 3H-DHA binding. These results suggest that choline and inositol in the membranes could have a crucial role in drug-receptor interaction.
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