Abstract
Objective To investigate the relationship between cholesterol, the dynamic of gallbladder and their role in cholesterol gallbladder formation by establishing an animal model of cholesterol gallstone. Methods The model of cholesterol gallstone was prepared by feeding guinea pig with stone-leading forage. The contents of serum total cholesterol (TC) and bile cholesterol (BC) of subgroup A [the normal guinea pigs], subgroup B [stone-leading forage for four weeks], subgroup C [stone-leading forage for eight weeks] were detected respectively. The effect of cholecystokinin-octopeptide (CCK-8) on constrictions of extracor-poreal gallbladder muscle strip was observed in subgroup A, B, C and D [gallbladder strip incubation in cho-lesterol 2 hours] to probe its possible mechanism. Results No gallstone was found in subgroup A. A total of 13 cholesterol gallstone guinea pigs was seen in subgroup B and group C. With the increase of TC and BC, the amplitude of contractions was upregulated by CCK-8 in a dose-independent manner in subgroup A, B, C and D (P<0.05). As compared with subgroup A, there was significant change in subgroup B, subgroup C and subgroup D (P<0. 05). The response of CCK-8 on the constrictions of extracorporeal gallbladder mus-cle strip could be inhibited partly by cholesterol (change 69.2%, P<0. 05). The effects of cholesterol on the constrictions of extracorporeal gallbladder muscle strip could be inhibited partially by atropine and nimo-dipine (changed for 64. 2%, 62. 1% respectively, P<0. 05). However, it could not be affected by TTX (P<0. 05). Conclusion With increase in the contents of TC and BC, gallbladder muscle strip tension and it's sensitivity to CCK-8 are decreased and the ratio of gallstone formation is increased. Cholesterol can pro-mote the formation of cholesterol stone by resulting in a defective gallbladder muscle strip contraction. Key words: Cholelithiasis; Cholesterol; Muscle strip
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