Abstract
The association between plasma cholesterol levels and the development of dementia continues to be an important topic of discussion in the scientific community, while the results in the literature vary significantly. We study the effect of reducing oxidized neuronal cholesterol on the lipid raft structure of plasma membrane. The levels of plasma membrane cholesterol were reduced by treating the intact cells with methyl-ß-cyclodextrin (MßCD). The relationship between the cell viability with varying levels of MßCD was then examined. The viability curves are well described by a modified form of the empirical Gompertz law of mortality. A detailed statistical analysis is performed on the fitting results, showing that increasing MßCD concentration has a minor, rather than significant, effect on the cellular viability. In particular, the dependence of viability on MßCD concentration was found to be characterized by a ~25% increase per 1 μM of MßCD concentration.
Highlights
The imbalance between the production of reactive oxygen species (ROS) and the biological system’s ability to detoxify the reactive intermediates leads to the oxidative stress [8]. Such stress has been implicated in the progression of major neurodegenerative diseases, namely, the Alzheimer disease (AD) and Parkinson’s disease [6]
Oxidative stress in cell biology refers to the inability of a cell or organism to handle ROS, either due to increased production of ROS or due to decreased antioxidant defense enzymes
Aerobic organisms have integrated antioxidant systems, which include enzymatic and nonenzymatic antioxidants, that are usually effective in blocking harmful effects of ROS
Summary
Dayeh 1,2,*, George Livadiotis F1i,gFuarreza8.n(LAemft)inDiiaangFri3ag,muKrsewo8fa.nt(hLHeemf.tC)oDdhiefaingegdra3mp,-vs aolfuethaenmd ordedifuiecdedp-cvhail-usqeuaanrde crehdauraccetder James L. Roberts 3, Nikita ViswasaFmigu3raenf8di.tt(SiLnaegbfteo) rfDEtihalegarypadmairsa3moffietttehinresgmαoof(dati)hf,ieβdp(pab-r)v,aamγlue(etce)ar,snadαndr(eadδ),u(cβde)d(,bw)c,hiγti-hs(qcMu),aßraCenDdchpaδlroa(dtct)te,edrwi,ziaitnhsgaMt. Received: 1 April 2020; Accepted: 17 Athpartilth20ep2ah0ry;aPpmuoetbhtleiesrshiβsedao:nf2db1eδAinhppgaragivdrloeae2omp0tdhe2ene0tedosrepsnβttoiamfonanfdiltMtvδinaßhlgCuaevDEaserqeioutshfaprettleiohoojtentpceptdie(-m9vd)aa.asllOwufvenuhacnialtlhuncehatedeirsioatornocetsitfhdseetuorhridfczeeehitdnphpagi-neccvdhttameh,ild-ueutsheqllateuiasnpacnelraadiaecsr paramettheur sβ, tahnedhδyphoatvheestihsetohfopupMasr,tißatmhmCeaDelhtedvyraeplαpoueteahnsneddsoeifnsγctohehfceaiMhsvpaeaß-rvcCatcahcDelteupedreotieepzadpitnn.iemdgnadrtlehvndegacuoleciuoneidesdesnaaecorchscffseiit-pthsotqteifenudpgfa-i.tvroteaifnlfugtohereaa│rnpebda/
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