Effects of chitosan, gallic acid, and algicide on the physiological and biochemical properties of Microcystis flos-aquae.

Abstract

The effects of chitosan, gallic acid, and algicide chitosan-gallate on the activities of antioxidant enzymes, malonaldehyde (MDA) content, and photosynthetic activity of Microcystis flos-aquae were investigated to explore the physiological and biochemical mechanisms of algicides. Results demonstrated that chitosan did not significantly affect catalase (CAT) and superoxide dismutase (SOD) activities, MDA content, and photosynthetic activity in this alga. At 30mgL(-1), gallic acid, CAT, and SOD activities and MDA of M. flos-aquae cells showed maximums of 2.872 × 10(-10)mg·cell(-1)min(-1), 0.787 × 10(-8)U·cell(-1), and 0.626 × 10(-8)nmol·cell(-1), respectively. Photosynthetic organs in algal cells were severely damaged under the stress of high gallic acid concentrations, inducing blockage of photosynthetic electron transport and resulting in the inability to restore normal photosynthetic activity. CAT and SOD activities and MDA content with lower algicide concentration were significantly higher than the control group (p < 0.05) and, in higher algicide groups, significantly lower than the control (p < 0.05). Algicide releasing gallic acid in groups treated with 60, 90, and 130mg/L algicide was strong enough to cause severe damage to photosynthetic organs in these algal cells. The algicide suppression time was longer than that of directly added gallic acid.