Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis

Abstract

In this study, the effect of chitosan (CH) and chitosan oligosaccharide (COS) on serum and tissue (liver, kidney, brain and testis) total sialic acid (TSA) level was investigated in rats with experimental fluorosis. The groups were formed as control, sodium fluoride (NaF), NaF+chitosan (NaF+CH), NaF+chitosan oligosaccharide (NaF+COS), chitosan (CH) and chitosan oligosaccharide (COS). Drinking water of NaF groups was prepared at a concentration of 100 ppm sodium fluoride. Chitosan and chitosan oligosaccharide were given to Experimental groups as 250 mg/kg dose by gastric gavage for ninety days. At the end of the study, TSA level was determined in serum, liver, kidney, brain and testicular tissues. Compared with the control group, it was found that TSA levels increased in serum, liver, kidney, brain and testis tissues in the group treated with sodium fluoride (p<0.05). According to the NaF group, there was a significant decrease in serum levels in the NaF+CH group and in the serum, liver and kidney tissues in the NaF+COS group. It was determined that there was no difference between the control and experimental groups in terms of brain tissue sialic acid level (p>0.05). In conclusion, it can be thought that fluorine intoxication causes an increase in TSA levels by causing cell damage in serum and tissues. In the study presented, CH and COS have been shown to reduce TSA levels. Also, in this study, COS was found to be more effective in reducing the TSA level.