Effects of chitin and sepia ink hybrid hemostatic sponge on the blood parameters of mice.

Yu-Lin Sun,Dao-Hai Chen,Wei Zhang

doi:10.3390/md12042269

Abstract

Chitin and sepia ink hybrid hemostatic sponge (CTSH sponge), a new biomedical material, was extensively studied for its beneﬁcial biological properties of hemostasis and stimulation of healing. However, studies examining the safety of CTSH sponge in the blood system are lacking. This experiment aimed to examine whether CTSH sponge has negative effect on blood systems of mice, which were treated with a dosage of CTSH sponge (135 mg/kg) through a laparotomy. CTSH sponge was implanted into the abdominal subcutaneous and a laparotomy was used for blood sampling from abdominal aortic. Several kinds of blood parameters were detected at different time points, which were reflected by coagulation parameters including thrombin time (TT), prothrombin time (PT), activated partial thromboplatin time (APTT), fibrinogen (FIB) and platelet factor 4 (PF4); anticoagulation parameter including antithrombin III (AT-III); fibrinolytic parameters including plasminogen (PLG), fibrin degradation product (FDP) and D-dimer; hemorheology parameters including blood viscosity (BV) and plasma viscosity (PV). Results showed that CTSH sponge has no significant effect on the blood parameters of mice. The data suggested that CTSH sponge can be applied in the ﬁeld of biomedical materials and has potential possibility to be developed into clinical drugs of hemostatic agents.

Highlights

Chitin is a homopolymer of 1–4 linked 2-acetamido-2-deoxy-β-D-glucopyranose, some of the glucopyranose residues are deacetylated and occur as 2-amino-2-deoxy-β-D-glucopyranose [1], in addition, chitin is the only alkaline polysaccharide in nature [2]
We found that CTSH sponge had no significant effects on the coagulation and fibrinolysis parameters of mice after being absorbed and degraded gradually in vivo during the testing time
Our findings showed that CTSH sponge had no significant effects on hemorheology of mice after being absorbed in vivo

Summary

Introduction

Chitin is a homopolymer of 1–4 linked 2-acetamido-2-deoxy-β-D-glucopyranose, some of the glucopyranose residues are deacetylated and occur as 2-amino-2-deoxy-β-D-glucopyranose [1], in addition, chitin is the only alkaline polysaccharide in nature [2]. Chitin and its derivatives are biodegradable and biocompatible biomedical materials to humans and most animals [3]. Previous reports have indicated that there are numerous studies and applications about chitin in hemostatic materials, drug delivery and tissue repairing materials [4,5,6,7,8]. The hemostatic mechanism of chitin includes two methods. One is that chitin and platelet are adhered to each other through protein-mediation, the composite of chitin/platelet is formed, which can accelerate the polymerization of fibrin monomers and form clots together. Chitin induces erythrocyte aggregation and stimulates vasoconstriction

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Conclusion