Effects of Changes of Diet from High‐fat to Low‐fat on Hepatic mRNA Levels of ACS, CPT‐1, and ACC

Abstract

AimsThe purpose of this study was to investigate the effects of dietary changes from high‐fat diet (HFD, 45% of total calories from fat) to low‐fat diet (LFD, 10% of total calories from fat) on hepatic mRNA levels of acyl coenzyme A synthetase(ACS), carnitine palmitoyltransferase‐1(CPT‐1), and acetyl coenzyme A carboxylase(ACC) as well as on serum and hepatic lipids in mice.MethodsFive weeks‐old male C57BL/6 mice were fed high‐fat diet for 9 weeks then switched to a low‐fat diet or continued the high‐fat diet for 3 weeks. Body weight, serum and hepatic lipid levels were measured. Expressions of ACS, CPT‐1, and ACC mRNA were also analyzed.ResultsThe results showed that after the high‐fat diets were switched to low fat diets, body weight, epididymal and retroperitoneal fats were significantly reduced (p<0.05). The levels of serum and hepatic total cholesterol were not different between the two groups however, serum TG levels of high‐fat diet followed by low‐fat diet group were significantly lower than those of high‐fat diet group (p<0.05). Hepatic mRNA levels of CPT‐1 and ACC were higher significantly in the group of high‐fat diet followed by low‐fat diet (p<0.05) however mRNA levels of ACS were not different between the two groups.ConclusionsChanges in the composition of dietary fat from high to low decreased significantly body fats and the serum TGs. Furthermore, changes of dietary fat composition affected differently the hepatic mRNA levels of ACS, CPT‐1 and ACC.