Effects of changes in intravascular oncotic pressure on renal responses to volume loading in the saltwater-acclimated Pekin duck (Anas platyrhynchos)

Abstract

The relative contributions of the intra-and extravascular compartments of the extracellular fluid (ECF) to the control of osmoregulatory renal functions were examined in saltwater-acclimated Pekin ducks. Having established steady-state diuresis and salt gland secretion by continuous infusion of 1 ml·min-1 isotonic Krebs-Ringer-Bicarbonate (KRB) solution, 5% dextran-70 was added to the infusate for 30 min thereby confining volume expansion to the intravascular compartment. General volume expansion by isotonic KRB caused a drop in plasma osmolality by 23 mOsm·kg-1, due to NaCl elimination by the salt glands, and decreases in hematocrit (het) and radioimmunologically measured plasma levels of Arg8-vasotocin (AVT) and Val5-angiotensin II (ANG II), whereas immunoreactivity associated with atrial natriuretic factor (ir-ANF) was increased. Adding 5% dextran-70 to the infusate left plasma osmolality and electrolytes unchanged but was followed by a further decrease in hct and a 36% increase in the plasma colloidosmotic pressure (COP) facilitating fluid shifts from the extra-to the intravascular compartment of the ECF. Plasma levels of AVT and ANG II remained unchanged, but ir-ANF rose three-fold, its increase being three times as great relative to the decrease in hct, as during general volume expansion by isotonic KRB solution. Arterial and central venous pressure measurements did not indicate changes in cardiovascular function. Hyperoncotic infusion initially induced marked antidiuresis with decreased osmolal excretion, despite a slightly elevated urine osmolality. This effects, however, was trasient and not proportional to the rise in COP, but rather seemed to be related to fluid shifts resulting from hyperoncotic loading. With tracer dilution techniques, reductions in both renal plasma flow and glomerular filtration rate were found to contribute to antidiuresis which was associated with reduced fractional water excretion. Salt gland secretion rate did not increase during hyperoncotic intravascular volume expansion but rather tended to decrease. The results of this study are in line with the idea that contributions of the interstitial fluid compartment (IFC) to volume-dependent control of osmoregulatory functions have to be considered. In the present study on saltwater-acclimated ducks, AVT, ANG II, and ir-ANF could be excluded as mediators of the adjustments in renal water and salt handling following fluid shifts due to hyperoncotic intravascular volume expansion.