Abstract
The study was aimed to identify the effective cervical ripening treatment to increase the pregnancy rates following transcervical artificial insemination (TC-AI) in indigenous ewes of Bangladesh. Three treatments schedules were compared, G1 (n = 6, control), G2 [n = 6, prostaglandin E1 (PGE1) as Misoprostol-Cytomis®] and G3 (n = 6, estrogen as Oestriol-Ovestin® + oxytocin as Linda-S® Vet). Ewes (n=18) were synchronized for estrus using two doses of 100 µg prostaglandin (PGF2α; Ovuprost®) 9 days apart. The cervical ripening treatments were administered just after the onset of estrus and left for 12 h before TC-AI. The cervical ripening was reflected with the depth of cervical penetration. The depth of cervical penetration in G1, G2 and G3 both before and after cervical ripening treatments were 0.33 ± 0.10 cm vs 0.43 ± 0.07 cm, 0.41 ± 0.09 cm vs 3.50 ± 0.26 cm and 0.43 ± 0.09 cm vs 1.54 ± 0.14 cm, respectively. The depth of cervical penetration increased significantly (p
Highlights
Nowadays, sheep farming is gaining popularity among Bangladeshi farmers
To evaluate the mass activity, a drop rates in Bangladeshi ewes after using cervical ripening (0.5 μl) of semen was placed on a pre-warmed slide treatments in the transcervical artificial insemination (TC-Artificial insemination (AI)) technique has been reported, (35°C) without coverslip and examined under a the objective of this study was to identify microscope equipped with phase-contrast optics the effective cervical ripening treatments on (100×)
The success of TC-AI depends on semen deposition into the cervix as deep as possible for increased pregnancy rates (>50%; Leethongdee, 2011)
Summary
Sheep farming is gaining popularity among Bangladeshi farmers. Farmers are looking for sustainable and locally appropriate reproductive techniques to upgrade their sheep genetics. Artificial insemination (AI) in conjugation with estrus synchronization is extensively used in sheep breeding programs to disseminate genetic traits of interest and increase production. Achieving acceptable pregnancy rates is very important for uptake to AI technology by sheep farmers. To establish an AI program in Bangladeshi ewes, studies on estrus synchronization, AI techniques using chilled and frozen. Volume 4, Issue 2, pp: 84-89 semen have been accomplished (Roy et al, 2014; Jha synchronized for estrus using prostaglandin (PGF2α) et al, 2020). The pregnancy rates reported before underwent cervical ripening treatment.
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