Abstract
Stem cells nanotechnology has emerged as a new exciting area, and hold s great potential for research and development of stem cells as novel therapeutic platforms for genetic, traumatic, and degenerative medicine . Vital to the success of this technology are approaches that reproducibly facilitate in vivo cell tracking, expansion, differentiation, and transplantation. Herein we reported the effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes (FMNTs) on mice embryonic stem cell line CCE cells. The FMNTs were prepared by plasma surface treatment and characterized by high resolution transmission electron microscopy(HR-TEM), and incubated with murine ES CCE cells for 1 to 28day.These ES cells were observed by confocal laser scanning microscopy, and were analyzed by real time reverse transcription-polymerase chain reaction(RT-PCR), flow cytometry(FCM) and MTT method . Results showed that prepared FMNTs exhibited green fluorescent signal, could enter into ES cells in time-dependent means , more than 20 m g/ml FMNTs induced ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth i n dose - and time -dependent means, induced apoptosis of ES cells; conversely, 5 m g/ml FMNTs could markedly stimulate the expression of Sox1 and Hsp27, and inhibit expression of OCT4 in ES cells, FCM analysis showed that differentiation marker Flk-1 exhibited higher expression compared with control ES cells. In conclusion, high dose of FMNTs can inhibit proliferation of ES cells, low dose of FMNTs can improve the differentiation of ES cells, FMNTs can have potential applications in in vivo tracking, imaging and regulation of the proliferation and differentiation of ES cells.
Highlights
Generating progenitor cells with in vivo reconstitution functions has been at the center of intense research to accelerate biomedical applications of embryonic stem cells (ES) for the treatment of debilitating genetic, traumatic, and degenerative diseases [1,2,3]
We developed a unique, multifunctional nanostructure consisting of multi-walled carbon nanotubes with surface-conjugated CdSe/ZnS quantum dots (FMNTs), and investigated the effects of as-prepared FMNTs on murine embryonic stem cells with the aim of developing a new method to regulate proliferation and differentiation of ES cells based on nanomaterials
Acrylic acid-coated, multiwalled carbon nanotubes with surface-conjugated QDs represent a unique nanostructured material that efficiently accumulates in mouse embryonic stem cells rendering them highly fluorescent
Summary
Generating progenitor cells with in vivo reconstitution functions has been at the center of intense research to accelerate biomedical applications of embryonic stem cells (ES) for the treatment of debilitating genetic, traumatic, and degenerative diseases [1,2,3]. A major challenge for clinical development of these pluripotent cells is effective, non-invasive imaging of transplanted cells to monitor biodistribution (i.e., in vivo tracking). Reproducible approaches need to be developed enabling efficient intracellular delivery of biomolecules, including. DNA, RNA and proteins, required to control ES cell differentiation. Physical methods such as electroporation and nucleofection offer the advantage of high delivery efficiency but frequently cause severe damage to ES cells [4]. Viral vectors, including retro-, lenti-, and adenoviruses, result in successful transfection and reproducible manipulation of ES differentiation in vitro. Risk of toxicity, immunogenicity, and increased mutagenesis significantly decrease clinical viability of these viral carriers for biomedical application
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
