Abstract
The effects of haevy metals (Cd2+, Cu2+, Pb2+ and Zn2+) on activities of carp trypsin, α‐chymotrypsin, carboxypeptidase A and lipase were studied. The enzymes were isolated from the gastrointestinal tract and the effects of metal ions were investigated during incubation for 5 min. The presence of Cd2+ did not influence the activities of CPA and trypsin and 10–20 % inhibiton was observed with α‐chymotrypsin and lipase. Cu2+ only slightly influenced the trypsin and lipase activities, whereas the α‐chymotrypsin activity was decreased. All enzyme activities decreased at higher Zn2+ concentrations. The inhibition was most pronounced at α‐chymotrypsin (more than 50%). With the exception of trypsin, Pb2+ inhibited the activities of the investigated enzymes.
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