Abstract
The gonadotropic potency of purified carp gonadotropin (c-GTH) was tested by evaluating ovarian maintenance, maturation, and ovulation during the spawning season in the hypophysectomized gravid catfish, Heteropneustes fossilis. Daily treatment with c-GTH (0.5, 1.0, and 2.5 μg/fish or 10, 20, and 40 ng/g body weight) for 7 days significantly maintained the yolky oocytes in the gravid ovaries. c-GTH also evoked a dose-dependent maturation and ovulation response; catfish injected with 50 or 100 μg (or 0.55 or 1 μg/g body weight) ovulated a large number of ripe eggs. The present data show that c-GTH acts as a potent gonadotropin in the catfish.
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