Effects of Carboxymethyl-Lysine in Heat Processed Foods on the Plasma Metabolome in Mice

Abstract

Effects of Carboxymethyl-lysine in Heat Processed Foods on the Plasma Metabolome in Mice by Zhou Fang, Master of Science Utah State University, 2016 Major Professor: Dr. Robert E. Ward Department: Nutrition Dietetics and Food Sciences The effect of dietary carboxymethyl-lysine (CML) on the metabolite profile of plasma was investigated. Mice were fed one of five diets including: AIN93 diet (negative control), a 45% kcal from fat Diet Induced Obesity diet (DIO; positive control); CML0, Total Western Diet (TWD) with low CML; CML1, TWD with medium CML, and CML2, TWD with high CML. In addition, plasma glucose across the five diet groups was also quantitatively measured in this study. According to the results, 93 compounds were detected in the mouse plasma samples using Gas Chromatography-Mass Spectrometry (GC-MS). Among all 93 detected compounds, 49 of them were amino acids, fatty acids, organic acids, or other organic molecules, while 44 of them could not be identified and were considered to be “unknowns”. Four identified metabolites and 10 unknown metabolites were significantly